化學(xué)交聯(lián)結(jié)合質(zhì)譜技術(shù)(chemical cross-linking coupled with mass spectrometry,CXMS)技術(shù)在近十年內(nèi)得到了蓬勃發(fā)展。被廣泛應(yīng)用于蛋白質(zhì)結(jié)構(gòu)鑒定和蛋白質(zhì)相互作用分析。目前CXMS技術(shù)在實際應(yīng)用存在一定的局限性:1、復(fù)雜樣品處理效率偏低;2、能夠提供的交聯(lián)信息有限。該技術(shù)發(fā)展的最大瓶頸已經(jīng)體現(xiàn)在有限的化學(xué)交聯(lián)劑選擇和交聯(lián)效率上。百靈威作為專業(yè)供應(yīng)商,提供新型化學(xué)交聯(lián)劑Leiker和d6-Leiker,具有可富集、可切斷、可定量的作用[1]。
■ Leiker具有高效的富集功能,比傳統(tǒng)交聯(lián)劑有更高的效率
Figure 1. Leiker(2138035) allowed near 100% enrichment of target peptides from a cross-linked ten-protein mixture diluted with increasing amounts of non-cross-linked E. coli lysates[1].
■ Leiker可以顯著提高所能鑒定到的交聯(lián)肽段數(shù)量
Figure 2. CXMS analyses of E. coli and C. elegans lysates. The best protein-protein interaction cluster extracted from the Leiker-identified or BS3(146482)-identified (Yang et al., 2012) [2]inter-links from E. coli whole-cell lysates[1].
■ Leiker可以進行定量CXMS研究
Figure 3. Quantitative CXMS analysis of the L7Ae-RNA complex. Reciprocal labeling of RNA-free (F) and RNA-bound (B) L7Ae with [d0]/[d6]-Leiker(2322681)[1].
References
[1] Tan D, Li Q, Zhang M J, et al. Trifunctional cross-linker for mapping protein-protein interaction networks and comparing protein conformational states[J]. Elife Sciences, 2016, 5.
[2] Yang B, Wu Y J, Zhu M, et al. Identification of cross-linked peptides from complex samples.[J]. Nature Methods, 2012, 9(9):904-906.
■ 新型蛋白質(zhì)交聯(lián)劑
營銷編號
|
產(chǎn)品名稱
|
中文名稱
|
包裝規(guī)格
|
2138035
|
Leiker, 95%
|
賴氨酸特異性可富集型交聯(lián)劑
|
5 mg
10 mg
|
2322681
|
d6-Leiker, 95%
|
d6-賴氨酸特異性可富集型交聯(lián)劑
|
5 mg
10 mg
|
■ 其他交聯(lián)劑系列產(chǎn)品
產(chǎn)品編號
|
產(chǎn)品名稱
|
CAS
|
包裝規(guī)格
|
350636
|
4-Benzoylbenzoic acid N-succinimidyl ester, 97%
|
91990-88-4
|
50 mg
250 mg
|
146482
|
Bis(sulfosuccinimidyl) suberate sodium salt, BS3, 90%
|
82436-77-9
|
25 mg
100 mg
|
296329
|
Di(N-succinimidyl) 3,3'-dithiodipropionate, DTSP, 97%
|
57757-57-0
|
1 g
5 g
|
456454
|
Di(N-succinimidyl) glutarate, DSG, 97%
|
79642-50-5
|
50 mg
250 mg
|
253405
|
Di(N-succinimidyl) suberate, DSS, 98%
|
68528-80-3
|
1 g
5 g
|
1410146
|
Disuccinimidyl tartrate, DST, 95%
|
N/A
|
5 mg
25 mg
|
792543
|
3,3'-dithiobispropionic acid bis-sulfosuccinimidyl ester, DTSSP, 90%
|
81069-02-5
|
25 mg
100 mg
|
444282
|
N-Succinimidyl iodoacetate, 95%
|
39028-27-8
|
100 mg
500 mg
|
551313
|
Succinimidyl 3-(2-Pyridyldithio)Propionate, SPDP, 97%
|
68181-17-9
|
100 mg
|
■ 相關(guān)產(chǎn)品
產(chǎn)品編號
|
英文名稱
|
中文名稱
|
CAS
|
649777
|
Acrylamide/?Bis-?acrylamide Solution, 29:1, 40%
|
丙烯酰胺/雙丙烯酰胺溶液
|
N/A
|
398539
|
Aprotinin, 6000 U/mg
|
抑肽酶
|
9087-70-1
|
3922801
|
BCA Protein Assay Macro Kit
|
蛋白質(zhì)定量試劑盒
|
N/A
|
266630
|
Benzamidine hydrochloride, 99%
|
苯甲脒鹽酸鹽
|
1670-14-0
|
1914018
|
CAPS Buffer
|
CAPS 緩沖液
|
N/A
|
327499
|
Coomassie Brilliant Blue G
|
考馬斯亮藍G
|
6104-58-1
|
415951
|
Dithiothreitol, 99%
|
二硫蘇糖醇
|
3483-12-3
|
1914020
|
Phosphate Buffered Saline, 10×
|
PBS緩沖液
|
N/A
|
3910101
|
Protease Inhibitor Mix G
|
Cocktail蛋白酶抑制劑G
|
N/A
|
3910201
|
Protease Inhibitor Mix M
|
Cocktail蛋白酶抑制劑M
|
N/A
|
1201851
|
Protein Color Marker, M.W. 1,200 - 31,000 Da
|
蛋白預(yù)染Marker
|
N/A
|
1914019
|
SSC Buffer, 20×
|
SSC 緩沖液
|
N/A
|
1913953
|
Tris-Glycine-SDS Running Buffer, 10×
|
Tris-Glycine-SDS電泳緩沖液
|
N/A
|
954773
|
Trypsin, 2500 units/mg
|
胰蛋白酶
|
9002-07-7
|
■ 其他配套產(chǎn)品: