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      化學(xué)交聯(lián)結(jié)合質(zhì)譜技術(shù)(chemical cross-linking coupled with mass spectrometry,CXMS)技術(shù)在近十年內(nèi)得到了蓬勃發(fā)展。被廣泛應(yīng)用于蛋白質(zhì)結(jié)構(gòu)鑒定和蛋白質(zhì)相互作用分析。目前CXMS技術(shù)在實際應(yīng)用存在一定的局限性:1、復(fù)雜樣品處理效率偏低;2、能夠提供的交聯(lián)信息有限。該技術(shù)發(fā)展的最大瓶頸已經(jīng)體現(xiàn)在有限的化學(xué)交聯(lián)劑選擇和交聯(lián)效率上。百靈威作為專業(yè)供應(yīng)商,提供新型化學(xué)交聯(lián)劑Leiker和d6-Leiker,具有可富集、可切斷、可定量的作用[1]。

 Leiker具有高效的富集功能,比傳統(tǒng)交聯(lián)劑有更高的效率

Figure 1. Leiker(2138035) allowed near 100% enrichment of target peptides from a cross-linked ten-protein mixture diluted with increasing amounts of non-cross-linked E. coli lysates[1].

 Leiker可以顯著提高所能鑒定到的交聯(lián)肽段數(shù)量

Figure 2. CXMS analyses of E. coli and C. elegans lysates. The best protein-protein interaction cluster extracted from the Leiker-identified or BS3(146482)-identified (Yang et al., 2012) [2]inter-links from E. coli whole-cell lysates[1].

 Leiker可以進行定量CXMS研究

Figure 3. Quantitative CXMS analysis of the L7Ae-RNA complex. Reciprocal labeling of RNA-free (F) and RNA-bound (B) L7Ae with [d0]/[d6]-Leiker(2322681)[1].

References

[1] Tan D, Li Q, Zhang M J, et al. Trifunctional cross-linker for mapping protein-protein interaction networks and comparing protein conformational states[J]. Elife Sciences, 2016, 5.

[2] Yang B, Wu Y J, Zhu M, et al. Identification of cross-linked peptides from complex samples.[J]. Nature Methods, 2012, 9(9):904-906.

新型蛋白質(zhì)交聯(lián)劑

營銷編號

產(chǎn)品名稱

中文名稱

包裝規(guī)格

2138035

Leiker, 95%

賴氨酸特異性可富集型交聯(lián)劑

5 mg
10 mg

2322681

d6-Leiker, 95%

d6-賴氨酸特異性可富集型交聯(lián)劑

5 mg
10 mg

■ 其他交聯(lián)劑系列產(chǎn)品

產(chǎn)品編號

產(chǎn)品名稱

CAS

包裝規(guī)格

350636

4-Benzoylbenzoic acid N-succinimidyl ester, 97%

91990-88-4

50 mg
250 mg

146482

Bis(sulfosuccinimidyl) suberate sodium salt, BS3, 90%

82436-77-9

25 mg
100 mg

296329

Di(N-succinimidyl) 3,3'-dithiodipropionate, DTSP, 97%

57757-57-0

1 g
5 g

456454

Di(N-succinimidyl) glutarate, DSG, 97%

79642-50-5

50 mg
250 mg

253405

Di(N-succinimidyl) suberate, DSS, 98%

68528-80-3

1 g
5 g

1410146

Disuccinimidyl tartrate, DST, 95%

N/A

5 mg
25 mg

792543

3,3'-dithiobispropionic acid bis-sulfosuccinimidyl ester, DTSSP, 90%

81069-02-5

25 mg
100 mg

444282

N-Succinimidyl iodoacetate, 95%

39028-27-8

100 mg
500 mg

551313

Succinimidyl 3-(2-Pyridyldithio)Propionate, SPDP, 97%

68181-17-9

100 mg

相關(guān)產(chǎn)品

產(chǎn)品編號

英文名稱

中文名稱

CAS

649777

Acrylamide/?Bis-?acrylamide Solution, 29:1, 40%

丙烯酰胺/雙丙烯酰胺溶液

N/A

398539

Aprotinin, 6000 U/mg

抑肽酶

9087-70-1

3922801

BCA Protein Assay Macro Kit

蛋白質(zhì)定量試劑盒

N/A

266630

Benzamidine hydrochloride, 99%

苯甲脒鹽酸鹽

1670-14-0

1914018

CAPS Buffer

CAPS 緩沖液

N/A

327499

Coomassie Brilliant Blue G

考馬斯亮藍G

6104-58-1

415951

Dithiothreitol, 99%

二硫蘇糖醇

3483-12-3

1914020

Phosphate Buffered Saline, 10×

PBS緩沖液

N/A

3910101

Protease Inhibitor Mix G

Cocktail蛋白酶抑制劑G

N/A

3910201

Protease Inhibitor Mix M

Cocktail蛋白酶抑制劑M

N/A

1201851

Protein Color Marker, M.W. 1,200 - 31,000 Da

蛋白預(yù)染Marker

N/A

1914019

SSC Buffer, 20×

SSC 緩沖液

N/A

1913953

Tris-Glycine-SDS Running Buffer, 10×

Tris-Glycine-SDS電泳緩沖液

N/A

954773

Trypsin, 2500 units/mg

胰蛋白酶

9002-07-7

其他配套產(chǎn)品

百靈威高效酶制劑

百靈威熒光試劑特異性強的染色劑

百靈威新型生化染色劑
和染料

百靈威核心生化試劑

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